Features:-Peroxidaseenzymedetectionsystem-Elite®Avidin/biotincomplex(ABC)technology-Ready-to-Use-Noantibodyincluded-Highsensitivity/lowbackground-Approximately5timesmoresensitive-Containssufficientreagenttostainapproximately250-500sections-UsedasasecondlabelinmultipleantigenlabelingapplicationsKitContents:-50mlready-to-usestABIlizedElite®ABCreagentApplications:-Immunohistochemistry-Immunocytochemistry-Insituhybridization-BlottingApplications-Elispot-ELISADetails:TheVECTASTAIN®Elite®ABC-HRPKit(Peroxidase,Standard)ispartofthemodularVECTASTAIN®ABCsystem(seetheVECTASTAIN®ABCSystemdocument),whichcanbeusedtodetectanymoleculethatisbiotinylated.Advancedavidin/biotintechnology:TheElite®ABCcomplexissmaller,veryuniform,andhighlyactive,allowingmoreaccessibilityforbindingtoabiotinylatedtarget.TheElite®ABCseriesisapproximately5timesmoresensitivethantheoriginalVECTASTAIN®ABCKitwiththesamelowbackground.ThisStandardkitconsistsoftheReady-to-UseformulationoftheElite®HRPEnzymeConjugatereagentslistedinthefollowingstandardImmunohistochemistryprotocolonly,andisintendedtobepairedwithbiotinylatedsecondaryantibodies,suchasLS-D1(goatanti-rabbitIgG),LS-D2(horseanti-mouseIgG),orLS-D3(horseanti-goatIgG).Alternatively,thiskitisavailablepre-packagedwithvariouscombinationsofspeciesspecificblockingserumandbiotinylatedsecondaryantibody(seeVECTASTAIN®Elite®ABC-HRPStainingKitsLS-J1019thoughtLS-J1025).Immunohistochemistryprotocol:Fixation–Histology-AntigenRetrieval–EndogenousBlock–SerumBlock--PrimaryAntibody-SecondaryAntibody–EnzymeConjugate–Substrate-Counter-stain–Mounting